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免疫层析试纸划液仪的研制.doc

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免疫层析试纸划液仪的研制.doc
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免疫层析试纸划液仪的研制王清,郭雅婕(河北工业大学控制科学与工程学院,天津 300130)摘要:针对免疫层析检测试纸 C/T 线在生产制作上存在的不足,设计了一套对恒线速度运动的 NC 膜进行划液的专业设备,主要用于在试纸的 NC 膜表面划上定量均匀的蛋白质液。为了满足划液要求,划液仪以单片机为主要控制核心,设计了自主调节的划液平台和精确稳定的传动装置,通过键盘和液晶显示使操作更加简单直观,检测装置增加了划液仪的精度和稳定性。另外加入 RS-485 通讯接口, PC 机可通过 Modbus 协议实现对划液仪的控制,便于设备组网后的集中监控。本文提出了一种由双单片机控制的高性价比划液仪。讨论采用最方便实现物液设备应具备的所有基本功能,并从软件硬件两个方面入手,分析系统中各个部分的关系。给出了软件和硬件的方案。并对触摸屏部分实现进行了详细地说明。实验证明:该仪器经济简便、性能稳定、操作精准,具有较高的划液精度和多种模式,智能化程度高,安全性好,适用于各种免疫层析试纸条的科研以及中小型企业的研发。关键词:免疫层析;划液仪;RS485 总线;传感器;触摸屏中图分类号:TP273 +.5 文献标志码: ADesign and Implementation of Dispenser Device For immunochromatographic stripWANG Qing, GUO Ya-jie(School of Control Science and Engineering, Hebei University of Technology, Tianjin 300130,China)Abstract: According to the shortage of the production of immunochromatographic strip C/T line, It designed a professional draw liquid equipment for a set of constant linear velocity movement of NC membrane, mainly used to draw quantitative protein solution in the strip of NC film surface. In order to meet the requirements, dispenser instrument used single chip microcomputer as main control core, manual adjusted the position platform marking the appropriate location and spacing. Designed the position platform of independently regulate and precise and stable gearing, through the keyboard and LCD display made operation more simple and intuitive, liquid detection device increased the precision and stability. Add the RS-485 communication interface, so PC could be easy to control the instrument through the Modbus protocol when it used in the network. Practice proves, the performance of the instrument is economical and simple, performance stable and operate accuracy. It can be applied to the research and the development of small and medium-sized enterprises.Key words: immunochromatographic; dispenser instrument; SCM; gearing; step motor0引言近年来免疫学快速诊断技术和检测方法得到了迅速的发展并广泛应用于各个领域。目前在医学检验中的应用主要是免疫层析法和快速免疫金渗滤法,用于检测HBsAg、HCG 和抗双链 DNA 抗体等,其中胶体金免疫层析技术凭借其快速、简便、精确、稳定等优点,已经成为近年来生物产业化最成功的产品之一 [1]。划液仪
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