• / 18
  • 下载费用:10 金币  

髓鞘少突胶质细胞糖蛋白MOG抗原肽免疫原性验证.doc

关 键 词:
髓鞘少突胶质细胞糖蛋白MOG抗原肽免疫原性验证.doc
资源描述:
XX大学毕业论文题 目:髓鞘少突胶质细胞糖蛋白 MOG 抗原肽免疫原性验证学生姓名: XXX 学号: XXXXXXXXX 指导教师: XXX 职称: 教授 专 业: 生物技术 院(系): 生物工程系 完成时间: 2011 年 5 月 25 日 2011 年 5 月 25 日摘要目的:通过实验性自身免疫性脑脊髓炎(Experimental autoimmune encephalomyelitis, EAE)小鼠模型的建立,验证固相合成法制备的髓鞘少突胶质细胞糖蛋白(MOG)抗原肽免疫原性。并通过观察 EAE 病理学变化,初步探讨多发性硬化(MS )的发病机制。方法:本实验室利用 Fmoc 固相合成法制备的 MOG35~55 抗原肽(纯度95%)加完全弗氏佐剂(CFA )免疫 C57BL/6 小鼠,观察小鼠发病情况,并采用双盲法进行神经功能评分。利用电子显微镜观察 EAE 小鼠的病理学变化,分别采用 HE 染色、Luxol Fast Blue髓鞘染色法评估脊髓中炎性细胞的浸润及髓鞘脱失的情况。结果:MOG35~55 成功诱导了 C57BL/6 小鼠 EAE 模型。免疫后 15 天起,小鼠开始陆续出现临床症状,至第 21 天发病率达 100%。电子显微镜下可见 EAE 小鼠脊髓中典型脱髓鞘现象,并伴随有典型的炎性细胞的浸润。结论:用本实验室合成的抗原肽 MOG35~55 诱导的 C57BL/6 小鼠 EAE 模型,发病率高,模型稳定,为进一步研究多发性硬化(multiple sclerosis, MS)的发病机制与治疗打下基础。关键词:髓鞘少突胶质细胞糖蛋白、实验性自身免疫性脑脊髓炎、动物模型、神经病理学IAbstractObjectiveThe model of experimental autoimmune encephalomyelitis(EAE) was established in female C57BL/6 mice to validate the immunogenicity of myelin oligodendrocyte glycoprotein (MOG), to observe pathological changes of EAE, and to explore the possible pathogenesis of multiple sclerosis(MS).MethodsEach mouse was injected s.c over flanks with MOG35-55, Mycobacterium H37Ra, and CFA, injected i.p with pertussis toxin. The clinical symptoms were observed and the pathological changes of EAE were studied with the electron microscopy. Consecutive sections were stained with hematoxylin/eosin(HE) and Luxol Fast Blue(LFB), to assess inflammation and demyelination.ResultsThe incidence of EAE in immunization animal reached 100%, most mice developed clinical EAE with in about 15 days of immunization MOG35~55 peptides. With Electron microscopy, inflammatory cells in white matter and demyelination were observed. And the component of axon is disappeared.ConclusionThe immunogenicity of MOG35~55 synthesized by the improved solid-phase synthesis method was identified by the induction of EAE in C57BL/6 mice with stable performance and high incidence, which may be helpful in exploring the mechanism and therapy of Multiple sclerosis(MS). Key
展开阅读全文
  微传网所有资源均是用户自行上传分享,仅供网友学习交流,未经上传用户书面授权,请勿作他用。
0条评论

还可以输入200字符

暂无评论,赶快抢占沙发吧。

关于本文
本文标题:髓鞘少突胶质细胞糖蛋白MOG抗原肽免疫原性验证.doc
链接地址:https://www.weizhuannet.com/p-6497719.html
微传网是一个办公文档、学习资料下载的在线文档分享平台!

微传网博客

网站资源均来自网络,如有侵权,请联系客服删除!

 网站客服QQ:80879498  会员QQ群:727456886

copyright@ 2018-2028 微传网络工作室版权所有

     经营许可证编号:冀ICP备18006529号-1 ,公安局备案号:13028102000124

收起
展开