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血清中视黄醇结合蛋白免疫透射比浊测定法[J].doc

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血清中视黄醇结合蛋白免疫透射比浊测定法[J].doc
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sunshinesunshine血清中视黄醇结合蛋白免疫透射比浊测定法王晓蓉(上海电力医院 上海 200052)摘要:目的 对视黄醇结合蛋白免疫透射比浊测定法进行方法学和临床应用评价。方法 采用免疫透射比浊测定法,根据 NCCLS 相关文件,对方法进行精密度、线性、准确性等评价及临床初步应用。 结果 批内 CV 1.5%~2.2% ,批间 CV 2.8%~3.7% ,日间 CV 2.6%~3.1% ,总 CV 4.5%~6.7% ,线性范围 0至 210 mg/L , r =0.9985 ,对照组血清视黄醇结合蛋白含量:49.5±11.9 mg/L;糖尿病肾病组:81.0±12.8 mg/L;慢性肾小球肾炎组:133.8±22.8 mg/L;急性肾小球肾炎组:175.4±45.2 mg/L;慢性肾功能衰竭:88.4±30.7;肾病组与对照组有显著差异(P〈 0.01) 。结论 免疫透射比浊法测定血清视黄醇结合蛋白,方法简便、快速、灵敏,结果准确,可用自动分析仪测试,适合临床检验科应用。对肾脏疾病的早期诊断有一定临床意义。关键词 视黄醇结合蛋白 免疫透射比浊法 肾功能Turbidimetric Immunoassay for determination of Retinol Conjugated Protein in SerumXiaorong Wang . ( Shanghai Power Hospital ,Shanghai, 200052,China)Abstract:Objective: To evaluate the methodology and clinical application of turbidimetric immunoassay for determination of retinol conjugated protein in serum. Method: According to NCCLS relative documents, evaluate the precision, linearity and accuracy of this turbidimetric immunoassay and try a clinical application. Results: within-lot CV 1.5%-2.2%, between-lot CV 2.8%-3.7%, between-day CV 2.6%-3.1%, total CV 4.5%-6.7%, linearity range 0-210mg/L, r = 0.99985. Quantity of retinol conjugated protein in Compared Group: 49.5±11.9mg/L; Diabetic Renopathy Group: 81.0±12.8mg/L; Chronic Glomerulonephritis Group: 133.8±22.8mg/L; Acute Glomerulonephritis Group: 175.4±45.2 mg/L; Chronic Renal Failure Group: 88.4±30.7mg/L. There’s obvious difference between the kidney disease Group and the Compared Group. (P 0.01) Conclusion: The method to determine retinol conjugated protein by turbimidetric immunoassay is simple, quick, sensitive, and accurate-resulted. So it can be applied on automated analyzer in clinical testing laboratory. Also it has certain clinical significance for the preliminary diagnosis.Key words: Retinol Conjugated Protein Turbidimetric Immunoassay Renal Function 人视黄醇结合蛋白(Retinol – binding Protein , RBP)分子量约 21,000 ,半衰期 3~12h,等电点PH4.4~4.8,由肝细胞合成,分布在人的血清、脑脊液、尿液等 [1]。在血液中,RBP 与视黄醇(VitA) 、甲状腺素转运蛋白按 1:1:1 结合,以复合物形式存在,能携带 90%视黄醇,从肝脏向周围
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