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Microbial Exopolysaccharises.ppt

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Microbial Exopolysaccharises.ppt
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Microbial Exopolysaccharises,,Introduction and Definition,Polysaccharides are classified on the basis of their main monosaccharide components and the sequences and linkages between them, as well as the anomeric configuration of linkages, the ring size (furanose or pyranose), the absolute configuration (D- or L-) and any other substituents present.,,Exopolysaccharides (EPS) are found external to the structural outer surface of the microbial cell. And it can be applied to describe polymers of very diverse composition and different physical types.,Source of Polysaccharides,Plant: ganoderma, lentinan, panax, krestin,tremella fucifamis AnimalMicroorganism:EPS,Bioactivity of Polysaccharides,Stimulate immunesystem:B,T,LAK,N,NK,Mφ Anti-tumor activity:Mφ,IL-1,TNF,LAK---85%-90%Anti-virus activity:ß –1,3 glucans ,HIV-CD4 Prevent senescence:CTL-LBP Anti-Hypercholesterol :6-8 same monosaccharide,Analysis and Structure Determination,Composition analysis:HPLC Structure determination: hydrolysis, periodate oxidation, GC-MS,FAB-MS etc. Molecular mass determination: relative molecular masses,Exopolysaccharide produce was one kind of natural nontoxic product,Classified of EPS Saccharidelinks biosynthesissupplybody ,acceptbody and transenzymebiosynthesis conditions:Ferment temperature:isoperenoid phosphatepH 6-7sugar and saltnitrogen,Exopolysaccharides Structure,HemopolysaccharidesHetersaccharides:5 factors,Biosynthesis,The biosynthesis of most EPSs closely resembles the process by which the bacterial wall polymers peptidoglycan and lipopolysaccharides are formed. Formation of the precursors for polysaccharide synthesis occurs within the cytoplasm.,,Carbohydrate Precursors,Other factors,Acyl precursors Methyl group Amino acids Inorganic substituents Lipid intermediates,,Energy requirementshigh demand for energy (in the form of ATP) Post-polymerisation modification Extracellular synthesis,,,,,Physiology and Industrial Production,Influence of medium composition Carbon and energy source
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